IIS 7 throwing 500 errors for static content

I have configured an app pool with an integrated pipeline and .Net 4 framework. I am using Asp.Net MVC 3 to present my site...

My controllers and views are presenting properly, but references to my static content are all returning sporadic 500 errors from IIS.

For example: A direct request to /content/style.css returns a 500 error. The detailed error summary message indicates the handler in use is the "StaticFile" and the error code is "0x80070070"

I have granted the 'Everyone' user read access to my /content folder and all child objects. I have granted the 'Everyone' user full control over my App_Data folder that contains my SqlCompact database.

Any ideas what could be causing my web service to throw these errors?

The problem with this configuration turned out to be read-write access to the contents App_Data folder. SqlCompact generated a SDF file that needed to be marked as accessible to the web process. Once that was available, IIS started responding properly.