SSRS 403 forbidden from .NET application

We have a .NET application that is already working in a number of development and production environments. One part opens SSRS reports, one new server is giving me this error when it tries to run. I have double checked the installation of our application and done a bunch of troubleshooting including reinstalling SSRS. Any suggestions on how to troubleshoot further?

System.Web.HttpUnhandledException (0x80004005): Exception of type 'System.Web.HttpUnhandledException' was thrown. ---> System.Net.WebException: The request failed with HTTP status 403: Forbidden.