Users cannot access web site hosted on IIS, configured with Windows Authentication ON

I’m currently developing a web site that uses windows authentication, with ApplicationPoolIdentity configured with Identity=Application Pool. I can connect by login with my administrator user when asked by the browser and it works perfect, but if any other user tries to access, the browser asks for the credentials 3 times and then it throws a 401 Error.

Test scenarios:

1. If the server is configured with Anonymous Authentication ON, everybody is able to open the page.

2. If I try to set the application pool Identity to a regular user account, IIS doesn’t recognize the user/password provided.

3. The folder C:\inetpub\ was set with full control to Everyone, but the users can’t connect anyway.

4. The folder C:\inetpub\ was set with full control to a specific user, but the user can’t connect anyway.

5. Following a recommendation from another post, the following section was added to the web.config :

<authorization>
<allow users="*"/>
</authorization>

The windows logs in the Event Viewer don’t store anything about a failed login.

thanks for your comments

Update

i enabled and add Failed Request Tracing Rules to web site

it was result

ModuleName :IIS Web Core

Notification : AUTHENTICATE_REQUEST

HttpStatus : 401

HttpReason : Unauthorized

ErrorCode : Access is denied (0x80070005)

i searched this error but the post tell me, maybe permissions, but i set everyone as full control and it doesn't working